Ozone (O3) is the second most powerful oxidant after fluorine (F2), and its germicidal efficacy is stronger than chlorine. Its bactericidal mechanism is to destroy the structure of microbial membrane by oxidation to achieve bactericidal effect.

Firstly,Ozone acts on the cell membrane, making the components of the membrane damaged, leading to metabolic disorders and inhibiting its growth. Ozone continues to penetrate through the membrane, damaging the lipoproteins and lipopolysaccharides in the membrane, changing the permeability of the cell, leading to cell dissolution until death.

This theory is in line with the actual effect that the ozone sterilization effect depends on the product of concentration C and time CT value and the increase of cell membrane thinning sterilization rate under high temperature. However, ozone inactivation of viruses is thought to be accomplished by the direct destruction of RNA or DNA by oxidation.

There are two kinds of oxidation reactions in ozone water sterilization. Microorganism not only reacts directly with ozone generator in dissolved water, but also indirectly with hydroxyl Oh - produced by ozone decomposition. Because hydroxyl Oh - is a highly oxidizing oxidant, the sterilization speed of ozone water is very fast.

Principle of ozone disinfection, sterilization and deodorization
Ozone sterilization belongs to biochemical oxidation reaction. Ozonation breaks down the enzymes necessary for glucose in bacteria.It can also directly interact with bacteria and viruses, destroy their cell walls and RNA, and decompose macromolecular polymers such as DNA, RNA, protein, lipids and polysaccharides.It can penetrate into the cell membrane and react with the lipopolysaccharide inside the cell membrane to the outer membrane lipoproteins, causing the permeability distortion of the bacterial substances, leading to the dissolution and death of the cells. It can also dissolve and denature the genetic genes, parasitic bacteria, parasitic virus particles, phages, mycoplasma and pyrogen (bacterial virus metabolites, endotoxin) in the dead bacteria.

Using the natural characteristics of ozone oxidation, the odor of amonia and organic pollutants produced in the decomposition of putrefaction is completely decomposed and removed. It destroys the molecular structure of odor producing substances and produces non-toxic and odorless substances.

Almost all international swimming pools are treated with ozone sterilization technology, which can not only remove the residual chlorine in the water, but also decompose the chloroform, bromodichloromethane, carbon tetroxide and other carcinogenic organic compounds produced by chlorine disinfection. Moreover, it can sterilize. It is the most economical way to extend the use time of pool water and reduce the cost.

Ozone sterilization technology has been widely used in manufacturing pharmacy and swimming pools for sterilization and disinfection due to its high efficiency, broad spectrum and no residue.